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Calculation for nosema spore count using a hemacytometer

calculation of nosema spore counts using hemacytometer

Randy Oliver, ScientificBeekeeping.com

From Cantwell 1970, assuming that each sampled bee is diluted with 1 mL of water.





Average spore count per bee is then:

Average count per single small square x 4,000,000.

Total count per large square (16 small squares) x 250,000.

Or total count per 5 large squares (80 small squares) x 50,000.

Category: Microscopy